This release introduces a major new connectome dataset and promotes a substantial collection of Split-GAL4 line datasets to production, alongside incremental updates to several existing datasets. The headline addition is the Berg2025 male CNS connectome, the largest single dataset addition in this release cycle.

New Datasets

Berg2025 — Male CNS Connectome (version 0.9)


Reference	Berg et al. (2025), doi:10.1101/2025.10.09.680999
Licence	CC-BY 4.0
Individuals	166,691
Ontology terms	10,316

Connectome neurons from the male Drosophila central nervous system (version 0.9). This dataset was not previously available in production and represents a significant expansion of VFB's male CNS connectomics coverage.

Note: NBLAST scores are not included in this release.

Split-GAL4 Line Datasets

The following Split-GAL4 line datasets are new to production in this release. All are licensed under CC-BY 4.0 unless otherwise noted.

Dataset	Reference	Individuals	Ontology Terms
`SplitShuai2023`	Shuai et al. (2023)	605	59
`SplitSterne2021`	Sterne et al. (2021), FBrf0251280	446	131
`SplitBogovic2020`	Bogovic et al. (2020), FBrf0247641	21	4
`SplitSchretter2020`	Schretter et al. (2020), FBrf0247707	34	5
`SplitMontague2019`	Montague et al. (2019), FBrf0233805	13	3
`SplitSitaraman2015`	Sitaraman et al. (2015), FBrf0230181	10	3
`SplitMasek2015`	Masek et al. (2015), FBrf0228601	3	2
`SplitJovanic2019`	Jovanic et al. (2019), FBrf0241547	4	1
`SplitFeng2020`	Feng et al. (2020), FBrf0247391	2	1
`SplitKind2021`	Kind et al. (2021), FBrf0252485	1	1
`SplitKlapoetke2022`	Klapoetke et al. (2022), FBrf0253524	1	1
`SplitNallasivan2025`	Nallasivan et al. (2025)	6	1

Updated Datasets

Dataset	Description	Change
`Aso2014`	MBONs and split-GAL4 lines that target them	+567 individuals (1,922 → 2,489)
`SplitSchretter2020`	Split-GAL4 lines from Schretter et al., 2020	+20 individuals (14 → 34)
`SplitWang2020a`	Split-GAL4 lines from Wang et al., 2020a	+6 individuals (49 → 55)
`Namiki2018`	split-GAL4 lines for descending neurons	+2 individuals, +1 ontology term
`Dorkenwald2023`	FlyWire connectome neurons	+3 ontology terms (7,493 → 7,496)
`Xu2020NeuronsV1point2point1`	JRC FlyEM Hemibrain neurons v1.2.1	+2 ontology terms (5,049 → 5,051)
`Dickson2017`	Dickson lab VT lines — FlyLight/Janelia images	+1 individual